HPLC Method for Analysis of Fenvalerate on Newcrom B Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-fenvalerateChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Fenvalerate
Fenvalerate is synthetic pyrethroid insecticide with the chemical formula C 25 H 22 ClNO 3 . It is used against a wide range of pests, partially due to it’s moderate mammalian toxicity following short-term and acute exposure. It is the most toxic to bees and fish. Upon contact, it may irritate skin and eyes.
You can find detailed UV spectra of Fenvalerate and information about its various lambda maxima by visiting the following link.
Fenvalerate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Insecticide |
| Analyzing Compounds | Fenvalerate |