HPLC Method For Analysis of Ethylene Glycol and Polyethylene Glycol (PEG) on Primesep AP Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylene glycol, PEG 200, PEG 400.
Polyethylene glycol ( PEG ) has a low toxicity and is used in a variety of products (creams, laxatives, lubricants). When attached to various protein medications, polyethylene glycol allows a slowed clearance of the carried protein from the blood. This makes for a longer acting medicinal effect, reduces toxicity, and allows longer dosing intervals.
HILIC separation can be used to determine polyethylene glycol content in various formulations using ELSD, RI detection, or LC/MS detection. HPLC separation of oligomers can be achieved by changing the amount of acetonitrile.
Method Parameters
| Mobile Phase | MeCN/H2O – 95/5% |
|---|---|
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, RI |
| LOD* | Ethylene glycol 6 ppm (UV) |
| Class of Compounds | Diol |
| Analyzing Compounds | Ethylene Glycol,Polyethylene Glycol |
HPLC Column Used
Primesep AP, 4.6 x 150 mm, 5 µm, 100 A, dual ended