HPLC Method for Separation of Ethyl Red and Crystal Violet on Chromni Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethyl red , Crystal Violet .

Ethyl red is a pH indicator with C 17 H 19 N 3 O 2 as its molecular structure. When the pH transitions from acidic to neutral, Ethyl red turns from yellow to red, hence the name. Outside of experiments, it is occasionally used as a dye in textiles and foods. You can find detailed UV spectra of Ethyl Red and information about its various lambda maxima by visiting the following link.

Crystal Violet ( Methyl Violet 10B ), another basic triarylmethane dye, has the C 25 H 30 ClN 3 . It is frequently used for histological stains and for identifying Gram-positive bacteria. It is said to have antibacterial, antifungal, and anthelmintic properties. It is a common component of navy blue and black inks in printing, inkjet printers, and ball-point pens. You can find detailed UV spectra of Crystal Violet and information about its various lambda maxima by visiting the following link.

Ethyl red , Crystal Violet can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and AmAC. Detection is performed using UV.

Method Parameters

HPLC Column Used