HPLC Method For Analysis of Ellagic Acid on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ellagic Acid
Ellagic acid ( EA ) is a naturally occurring polyphenol. The best sources of ellagic acid in the diet are pomegranate, strawberries, raspberries, blackberries, and walnuts. It has been investigated extensively because of its antiproliferative action in some cancers, along with its anti-inflammatory effects.
Ellagic acid can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HClO4) buffer. The analysis method can be UV detected at 253 nm.
Method Parameters
| Mobile Phase | MeOH/H2O – 40/60% |
|---|---|
| Buffer | HClO4 – 0.5% |
| Flow Rate | 0.2 ml/min |
| Detection | UV 253 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Ellagic acid |
HPLC Column Used
Newcrom BH, 2.1 x 100 mm, 5 µm, 100 A, dual ended