HPLC Method For Analysis Of Dopamine and Serotonin on Primesep 200 Column
https://sielc.com/hplc-method-for-analysis-of-dopamine-and-serotoninChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · High Performance Liquid Chromatography (HPLC) Method for Analysis of Dopamine and Serotonin
Dopamine is a key neurotransmitter and medical stimulant used to treat low blood pressure, low heart rate, and heart attacks. Serotonin is another key neurotransmitter that has pharmaceutical applications – namely as the key ingredient in antidepressant and anti-anxiety medications.These two neurotransmitters can be retained, separated, and analyzed on a Primesep 200 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be UV detected at 280 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 280 nm, |
| Class of Compounds | Neurotransmitters |
| Analyzing Compounds | Dopamine, Serotonin |