HPLC Method for Analysis of Diphenylamine on Newcrom R1 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-diphenylamineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Diphenylamine
Diphenylamine is an organic compound with the chemical formula (C 6 H 5 ) 2 NH . It is used primarily to distinguish DNA from RNA with the Dsiche test, as a scald inhibitor in apples, antioxidant, stabilizer of smokeless powder, and a redox indicator. It’s a derivative of aniline, while Metanil Yellow, Disperse Orange 1, and Acid Orange 5 are derivatives of Diphenylamine. You can find detailed UV spectra of Diphenylamine and information about its various lambda maxima by visiting the following link.
Diphenylamine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -60/40% |
|---|---|
| Buffer | H3PO4– 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Diphenylamine |