HPLC Method for Analysis of Dichloran on Obelisc R Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Dichloran
Dichloran , also known as 2,6-dichloro-4-nitroaniline , is a herbicide with the chemical formula C 6 H 4 Cl 2 N 2 O 2 . It is used primarily as a pre- and post-harvest gerbicide on various plants including but not limited to peaches, grapes, lettuce, celery, and more. It works through inhibiting hyphal growth, which delays fungal spore germination. You can find detailed UV spectra of Dichloran and information about its various lambda maxima by visiting the following link.
Dichloran can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -50/50% |
|---|---|
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 0.3 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Herbicide |
| Analyzing Compounds | Dichloran |