HPLC Method for Analysis of Desmosine
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Desmosine
Desmosine is pyridinium-based amino acid derivative with C 24 H 40 N 5 O 8 + molecular formula. It is found exclusively in elastin. Elevated levels of desmosine in urine can be a symptom of Chronic Obstructive Pukmonary Disease (COPF), so it can be used as a biomarker when monitoring progression and aiding in a potential diagnosis.
Desmosine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | AmAcetate – 30 mM pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 270nm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Desmosine |
HPLC Column Used
Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended