HPLC Method for Analysis of Deoxyuridine on Chromite™ Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-deoxyuridine-2Chromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Deoxyuridine
Deoxyuridine ( dU ) is an nucleoside with the C 9 H 12 N 2 O 5 molecular formula. While in living organisms it exists primarily to occasionally become a part of DNA, it’s variants, Idoxuridine and Trifluoride, work as antiviral drugs. If consumed, Deoxyuridine can be toxic.
Deoxyuridine can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 90% |
|---|---|
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Nucleotide Base |
| Analyzing Compounds | Deoxyuridine |
HPLC Column Used
Chromni, 4.6 x 150 mm, 5 µm, 100 A, dual ended