HPLC Method for Analysis of D-alpha-Tocopheryl acetate
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of D-alpha-Tocopheryl acetate
D-alpha-Tocopheryl acetate is a form of Vitamin E with a molucular formula of C 31 H 52 O 3 . It is also known as alpha-tocopheryl acetate. It is often used in cosmetics, food, and dietary supplements due to its antioxidant properties.
D-alpha-Tocopheryl acetate can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 98/2% |
|---|---|
| Buffer | H3PO4 – 0.02 % |
| Flow Rate | 2.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Vitamin, Hydrophobic |
| Analyzing Compounds | D-alpha-Tocopheryl acetate |
HPLC Column Used
Newcrom R1, 4.6 x 50 mm, 5 µm, 100 A, dual ended