HPLC Method for Analysis of Cystine on Primesep 100 Column

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Cystine · Cystine is an organic compound with the molecular formula C 6 H 12 N 2 O 4 S 2 .

Properties: Appearance: Typically appears yellowish with a waxy, compact, and partially opaque texture.

Molecular weight: ~240.3 g/mol · Solubility: Soluble in water with HCL or NaOH.

Uses: Plays a crucial role in various biological processes due to its ability to stabilize protein structures and its role as a precursor to the antioxidant glutathione.

Cystine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 200 nm.

Method Parameters

HPLC Column Used