HPLC Method for Analysis of Cysteine on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine .
L-Cysteine is an amino acid with the chemical formula C 5 H 10 N 2 O 3 . It is primarily a building block for protein, but it also has antioxidant effects. On occasion, it is used to support people dealing with cancer, diabetes, and hangover, but there is yet to be substantial evidence that it works. Poultry, egg, beef, and whole grains are rich sources of the amino acid. You can find detailed UV spectra of Cysteine and information about its various lambda maxima by visiting the following link.
Cysteine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 192nm |
| Limit Of Detection* | 4 ppm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Cysteine |