HPLC Method For Analysis Of Cysteine and Cystine on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-of-cysteine-and-cystineChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode · High Performance Liquid Chromatography (HPLC) Method for Analysis of Cysteine and Cystine
Cysteine is an amino acid that is an essential building block of a wide variety of proteins made and used throughout the entire body. Cystine is an oxidized dimer of cysteine that the body uses for redox reactions and as a linkage for proteins to keep their 3D structures. Cysteine and Cystine can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Cysteine, Cystine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended