HPLC Method For Analysis of Cyclodextrins on Primesep S2
https://sielc.com/hplc-method-for-analysis-of-cyclodextrins-on-primesep-s2Chromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of B-Cyclodextrin, G-Cyclodextrin and A-Cyclodextrin
Cyclodextrins are hexasaccharides that are natural starch-conversion products. Industrially, they are manufactured by enzymatic degradation of vegetable raw materials.
Alpha-cyclodextrin has the chemical formula C 36 H 60 O 30 . It is said to lower blood pressure, cholesterol, and glucose levels. It is primarily used as a dietary supplement for fiber.
Beta-cyclodextrin has the chemical formula C 42 H 70 O 35 . It is used in a large variety of industries primarily as a complexing agent. It’s most notable uses are in medicine as sustained release drugs carrier and to improve stability in drugs.
Gamma-cyclodextrin has the chemical formula C 48 H 80 O 40 . It is used as a complexing agent due to having the largest cavity size between the natural cyclodextrin. It has use across a variety of industries from food, to medicine, to textiles, and cosmetics.
Method Parameters
| Mobile Phase | MeCN- 70% |
|---|---|
| Buffer | AmFm pH 3.0- 20 mm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds | Sugar |
| Analyzing Compounds | beta-CYCLODEXTRIN,alpha-CYCLODEXTRIN,gamma-Cyclodextrin |