HPLC Method for Analysis of Creatine and Creatinine on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-creatine-creatinineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Creatine , Creatinine · Creatine is a non-protein amino acid-like compound with the molecular formula C 4 H 9 N 3 O 2 . · Properties: Appearance: Typically a white odorless powder. · Molecular weight: ~131.13 g/mol · Solubility: Soluble in water. · Uses: Dietary supplement. · Creatinine is a nitrogenous organic compound with the molecular formula C 4 H 7 N 3 O .
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | Phosphoric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | amino acid |
| Analyzing Compounds | Creatine,Creatinine |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended