HPLC Method for Analysis of Coumarin
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Coumarin
Coumarin is an aromatic chemical that is used as a fragrance ingredient. It has a sweet earthy scent and is typically found in Tonka beans, cinnamon, woodruff, bison grass, and green tea. Besides a pleasant smell it had anti-inflammatory, antibacterial, and antifungal properties.
Coumarin an be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | H3PO4 – 0.1 % |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 276 nm |
| Class of Compounds | Drug, Flavouring substance, Hydrophobic, Neutral |
| Analyzing Compounds | Coumarin |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 3 µm, 100 A, dual ended