HPLC Method for Determination of Climbazole in Dandruff Shampoo on Primesep B Column
https://sielc.com/hplc-method-for-analysis-of-climbazole-shampooChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Climbazole
Climbazole is an antifungal agent with the chemical formula C 15 H 17 ClN 2 O 2 . It is commonly used in hair care products, primarily in shampoos, to treat dandruff and seborrheic dermatitis. It works by inhibiting the growth of the fungi by interfering with ergosterol biosynthesis, which is a crucial component of fungal cell membranes. Without ergosterol, the fungal cell membrane becomes compromised, leading to the death of the fungus.
Climbazole can be retained, and analyzed on a Primesep B mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format a buffer. This analysis method can be detected in the UV 270 nm.
Method Parameters
| Mobile Phase | Gradient MeCN – 40-70%, 20 min |
|---|---|
| Buffer | Ammonium Formate pH 4.0-20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 275 nm |
| Class of Compounds | Allylamines |
| Analyzing Compounds | Climbazole |