HPLC Method for Analysis of Chromium (III) Ion on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Chromium(III) .
Chromium(III) is a nutrient with the chemical formula Cr 3+ . It is believed to be essential for human metabolism of insulin, sugar, and lipids. Though, the notion is currently contested. It can be found naturally in rocks, soil, plants, as well as various animal products.
Chromium(III) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of water and acetonitrile (MeCN) and TFA. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/10% |
|---|---|
| Buffer | TFA – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Limit Of Detection* | 3 ppm |
| Class of Compounds | Nutrient |
| Analyzing Compounds | Chromium(III) |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated