HPLC Method for Analysis of Choline on Obelisc R Column
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Description
· Separation type: Liquid Chromatography Ion Exclusion · High Performance Liquid Chromatography (HPLC) Method of Choline
Choline is a water-soluble vitamin-like essential nutrient. It is a constituent of lecithin, which is present in many plants and animal organs. Choline is a nutrient related to the B-vitamin family. First discovered in 1862, it wasn’t classified as an essential nutrient until very recently. It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Choline can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Formate (AmFm) buffer and detected by ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | AmFm pH 4.0- 5 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Drug, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Choline |