HPLC Method for Analysis of Chlorpyrifos (Dursban) on Newcrom B Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-chlorpyrifosChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorpyrifos-methyl (Dursban)
Chlorpyrifos , also known as Dursban , is a highly toxic organophosphate with the chemical formula C 9 H 11 Cl 3 NO 3 PS . It works through interrupting the electrochemical processes in nerves. This leads to a build-up of acetylcholine, which leads to paralysis and eventual death.
Chlorpyrifos-methyl (Dursban) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Pesticide |
| Analyzing Compounds | Chlorpyrifos-methyl (Dursban) |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended