HPLC Method for Analysis of Centralite on Newcrom R1 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-centraliteChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Centralite
Centralite is an organic compound with the chemical formula C 17 H 20 N 2 O . It is used primarily to stabilize smokeless powder. In forensics, it is detected by GunShot Residue analysis. It is used as fuel burns when a gun is fired. It can be found on a suspect or crime scene using gas chromatography-mass spectrometry.
Centralite can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -60/40% |
|---|---|
| Buffer | H3PO4– 0.1% |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Centralite |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended