HPLC Method for Analysis of Cagrilintide on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-cagrilintideChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Cagrilintide .
Cagrilintide is the an analogue of amylin with the chemical formula C 194 H 312 N 54 O 59 S 2 . It is currently under investigation as potential treatment for type 2 diabetes and obesity.
Cagrilintide can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| LOD* | 10.6 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Cagrilintide |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated