HPLC Method for Analysis of Caffeic Acid on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-caffeic-acidChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Caffeic acid
Caffeic acid is an organic compound with the formula (HO) 2 C 6 H 3 CH=CHCO 2 H . It plays a key role in scavenging reactive oxygen species (ROS) that are generated during energy metabolism. Caffeic acid is an active ingredient in caffenol, which is a photographic developer made from instant coffee. Pharmacologically, it is used as an antioxidant in vitro and also in vivo. It also shows immunomodulatory and anti-inflammatory activity. You can find detailed UV spectra of Caffeic acid and information about its various lambda maxima by visiting the following link.
Caffeic acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -20/80% |
|---|---|
| Buffer | H3PO4– 0.5% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Caffeic acid |