HPLC Method for Analysis of Butylated Hydroxyanisole (BHA) on Newcrom R1 Column on Cromite™
https://sielc.com/hplc-method-for-analysis-of-butylated-hydroxyanisoleChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Butylated Hydroxyanisole (BHA)
Butylated Hydroxyanisole ( BHA ) is a petrochemical with the chemical formula C 11 H 16 O 2 . Due to it’s antioxidant properties, it is used in preservation of food, cosmetics, and pharmaceuticals. Due to previous experiments in animals, it is suspected to be a carcinogen for humans.
Butylated Hydroxyanisole (BHA) can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Phenolic Antioxidants |
| Analyzing Compounds | Butylated Hydroxyanisole (BHA) |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended