HPLC Method for Analysis of Bromate Ion on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromate .
Bromate is an oxyanion with the chemical formula BrO 3 – . It is formed in water through a reaction of ozone and bromide. It is suspected to be a human carcinogen.
Bromate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 85/15% |
|---|---|
| Buffer | Ammonium Acetate pH 4 – 20 mM |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD |
| Limit Of Detection* | 0.5 ppm |
| Class of Compounds | Oxyanion |
| Analyzing Compounds | Bromate |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended