HPLC Method for Analysis of Brilliant Blue FCF (FD&C Blue No. 1) on Chromni Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Brilliant Blue FCF (Blue 1) .
Brilliant Blue FCF , also known as Blue 1 , is a triarylmethane dye with the chemical formula C 37 H 34 N 2 Na 2 O 9 S 3 . Dry, it has the appearance of a light-blue powder, but when dissolved in water, turns deeper blue . It is used primarily as a blue colorant in medication, food, and cosmetics. It is one of the oldest FDA-approved color additives, having been listed since the 1969. You can find detailed UV spectra of Brilliant Blue FCF (Blue 1) and information about its various lambda maxima by visiting the following link.
Brilliant Blue FCF (Blue 1) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ammonium acetate. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O |
|---|---|
| Buffer | AmAc pH 5.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | Vis 590 nm |
| Class of Compounds | Dyes |
| Analyzing Compounds | Brilliant Blue FCF (Blue 1) |