HPLC Method for Analysis of Biopterin on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-biopterinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Biopterin
Biopterin is a petrin derivative with the chemical formula C 9 H 11 N 5 O 3 . As a cofactor for aromatic amino acid hydroxylases, it is a part of the synthesis of neurotransmitters like dopamine, serotonin, and norepinephrine. A biopterin imbalance can lead to a variety of disorders, most of which lead to neurological problems.
Biopterin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Biopterin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended