HPLC Chiral Separation of Bifonazole on Cyralis CM Column
https://sielc.com/hplc-method-for-analysis-of-bifonazoleChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Bifonazole .
Bifonazole is an antifungal drug with the chemical formula C 22 H 18 N 2 . It is primarily sold as an ointment that works through inhibiting fungal ergostrol biosynthesis, which enables it to work against dermatophytes.
This application describes a normal-phase chiral HPLC method for the separation of Bifonazole enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
Bifonazole can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.
Method Parameters
| Mobile Phase | Hexane/Ethanol/DEA- 85/15/0.5% |
|---|---|
| Flow Rate | 1.0 mL/min |
| Detection | UV 280 nm |
| Resolution | 2.32 |
| Class of Compounds | Fungicide |
| Analyzing Compounds | Bifonazole |