HPLC Method for Analysis of Benztropine mesylate on Primesep SB Column
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Benztropine mesylate
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Benztropine methylsulfonate .
Benztropine is a medical drug used in treatment of patients with Parkinson’s disease. It works as an acetylcholine blocker decreasing muscle stiffness. Benztropine can be retained in HPLC on Primesep SB mixed-mode column by hydrophobic and anion-exchange properties. The isocratic method uses a mobile phase of acetonitrile (ACN) and water with sulfuric acid buffer and UV detection at 220nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 220 nm |
| Class of Compounds | Drug, Basic, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Benztropine methylsulfonate |
HPLC Column Used
Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended