HPLC Method for Analysis of Benzoic Acid on Newcrom BH Column on Alltesta™

Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Benzoic Acid

Benzoic Acid is an organic acid with antimicrobial properties.  It is typically used in cosmetics, food, and pharmaceuticals as a preservative against yeast and mold. As treatment, it is also used as a topical medicine of fungal skin infections. It can be naturally found in many berries and vegetable resins. With it’s chemical formula being C 7 H 6 O 2 , it is considered the simplest aromatic carboxylic acid. You can find detailed UV spectra of Benzoic Acid and information about its various lambda maxima by visiting the following link.

Benzoic Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with sulfuric acid as a buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used