HPLC Method for Analysis of B-Estradiol on Primesep 200 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of B-Estradiol
B-Estradiol is an estrogen steroid hormone. It is the major estrogen secreted by the premenopausal ovary. It is synthesized from testosterone primarily in the ovarian granulosa cells and placenta. B- estradiol is used as a medication, for instance in menopausal hormone therapy. Primesep 200, a reverse phase column , contains embedded acidic ionizable groups and can retain B-Estradiol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-50%, 5 min, 4 min hold |
|---|---|
| Buffer | Gradient H2SO4 – 0.05-0.3 %, 5 min, 4 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Hormone, Hydrophobic |
| Analyzing Compounds | B-Estradiol |
HPLC Column Used
Primesep 200, 3.2 x 100 mm, 5 µm, 100 A, dual ended