HPLC Method for Analysis of Ataciguat on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ataciguat .
Ataciguat is an drug with the chemical formula C 21 H 19 N 3 O 6 S 3 . It is primarily used as a guanylate cyclase activator. Through activating sGC and restoring the NO-cGMP pathways, it helps to reduce calcium deposition, slow valve calcification, and generally treats calcific aortic valve stenosis. t helps reduce calcium deposition You can find detailed UV spectra of Ataciguat and information about its various lambda maxima by visiting the following link.
Ataciguat can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50 – 80/20%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 241 nm |
| Limit Of Detection* | 150 ppb |
| Class of Compounds | Drug |
| Analyzing Compounds | Ataciguat |