HPLC Method for Analysis of Acetylsalicylic acid (ASA) on Primesep 100 Column on Alltesta™

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetylsalicylic Acid (Aspirin)

Acetylsalicylic acid (ASA) , also known as Aspirin , is an organic compound with the molecular formula C 9 H 8 O 4 . It is a nonsteroidal anti-inflammatory drug (NSAID) that is used to reduce fever, pain, and inflammation. It is also used to treat Kawasaki disease, pericarditis, and rheumatic fever. Aspirin is a genetic medication that is widely available and can be procured without a prescription. It is one of the oldest NSAIDs in the world.

Acetylsalicylic Acid (Aspirin) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.

Method Parameters

Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate1.0 mL/min
DetectionUV 275 nm
Class of CompoundsDrugs
Analyzing CompoundsAcetylsalicylic Acid (Aspirin)

HPLC Column Used

Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended