HPLC Method for Analysis of Ascorbyl Phosphate on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium ascorbyl phosphate .
Sodium Ascorbyl Phosphate is a popular, more mild, form of Vitamin C with the chemical formula C 6 H 6 Na 3 O 9 P . It is found in a variety of dermatology products and used for its antioxidant properties.
Sodium ascorbyl phosphate can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and either Sulfuric acid (H2SO4) or Phosphoric acid (H3PO4) as the ionic modifier. This analytical method can be UV detected at 238 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 or H3PO4 |
| Flow Rate | 0.5 ml/min |
| Detection | UV 238 nm |
| LOD | 0.05 ppm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Sodium ascorbyl phosphate |
HPLC Column Used
Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended