HPLC Method for Analysis of Ascorbic Acid on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ascorbic Acid
Ascorbic Acid is a vitamin with the molecular formula C 6 H 8 O 6 . Typically, it is used to treat scurvy, support immune system, and preserve food. It is a white to light yellow powder that is easily dissolved in water. It can be found in a large variety of fruits and vegetables, especially in citrus.
Ascorbic Acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 250 |
| LOD*UV | 25 ppb |
| Class of Compounds | Vitamin |
| Analyzing Compounds | Ascorbic Acid |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended