Analysis of Eliquis (Apixaban) on Primesep B Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-apixabanChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Apixaban
Apixaban is an anticoagulant medication with the chemical formula C 25 H 25 N 5 O 4 . It is used primarily to treat and prevent blood clots and stroke. It works through directly inhibiting factor Xa. In patients who have undergone knee or hop replacement surgery, apixaban helps prevent deep vein thrombosis.
Apixaban can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with phosphoric acid as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 30% |
|---|---|
| Buffer | Ammonium Formate |
| Flow Rate | 1.0 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Apixaban |
HPLC Column Used
Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended