HPLC ELSD Method for Analysis of Aminomethylphosphonic acid (AMPA) on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-ampaChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Aminomethylphosphonic acid (AMPA) .
Aminomethylphosphonic Acid is an aminophosphonate with the chemical formula CH 6 NO 3 P . It is primarily used as a biocide and pesticide, as well as in research to assess the exposure of glyphosate. It is a major toxic metabolite of glyphosate as is considered to be a similar toxicological concern. You can find detailed UV spectra of Aminomethylphosphonic acid (AMPA) and information about its various lambda maxima by visiting the following link.
Aminomethylphosphonic acid (AMPA) can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocraticmethod with a simple mobile phase consisting of water and acetonitrile (MeCN) and TFA. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN/H2O – 80/20% |
|---|---|
| Buffer | TFA – 0.2% |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| Limit Of Detection* | 300 ppm |
| Class of Compounds | Acid |
| Analyzing Compounds | Aminomethylphosphonic acid (AMPA) |