HPLC Method for Analysis of Amoxicillin on Primesep 100 Column with Cromite™
https://sielc.com/hplc-method-for-analysis-of-amoxicillinChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin
Amoxicillin is a prescription antibiotic with the molecular formula C 16 H 19 N 3 O 5 S . It is typically used to treat bacterial infections and is taken orally.
Amoxicillin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Amoxicillin |
HPLC Column Used
Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended