HPLC Method for Analysis of Ammonium Picrate on Obelisc N Column

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Chromatogram

Method Parameters

Mobile PhaseMeCN/H2O – 50/50%
BufferAmAc pH 4.0- 5 mM
Flow Rate1.0 ml/min
DetectionUV, 250 nm
Class of CompoundsExplosive, Hydrophobic, Ionizable, Base
Analyzing CompoundsAmmonium Picrate

HPLC Column Used

Obelisc N, 2.1×100 mm, 5 µm, 100A
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