HPLC Method For Analysis Of Alprazolam on Newcrom R1 Column
https://sielc.com/hplc-method-for-analysis-of-alprazolamChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Alprazolam
Alprazolam, also known as Xanax, is a perscription medication with the C 17 H 13 ClN 4 molecular formula. It is typically used to treat anxiety and panic disorders. It works through enhancing the effects of GABA, which is a calming neurotransmitter in the brain.
Alprazolam can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H3PO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds | Drug, Sedative, Hydrophobic, Neutral, Acid |
| Analyzing Compounds | Alprazolam |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended