HPLC Method for Analysis of Allantoin on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin
Allantoin is found in plants such as wheat sprouts, chamomile, sugar beet, and comfrey. It is used as a moisturizer, which is, as the name suggest, used to moisturize the skin and to treat or prevent skin irritations and dry skin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Allantoine. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | TFA- 0.1% |
| Flow Rate | 1.0ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Plant-based compound |
| Analyzing Compounds | Allantoin |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 3 µm, 100 A, dual ended