HPLC – MS Method for Analysis of Alfuzosin on Primesep 200 Column
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HPLC- MS Method for Analysis ofAlfuzosinonPrimesep 200Column
Description
· HPLC- MS Method for Analysis of Alfuzosin on Primesep 200 Column
Alfuzosin is a medication with the chemical formula C 19 H 27 N 5 O 4 . It is used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently. You can find detailed UV spectra of Alfuzosin and information about its various lambda maxima by visiting the following link.
Alfuzosin can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 247 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
Method Parameters
| Mobile Phase | MeCN – 70%, |
|---|---|
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 247 nm, SIM 390 + |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
| Class of Compounds | Quinazolines, alpha-1 adrenergic receptor antagonist |
| Analyzing Compounds | Alfuzosin |