HPLC Method for Analysis of Aconitic Acid on Chromni Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Aconitic acid , trans-Aconitic acid .
Aconitic acid is an organic compound with the chemical formula C 6 H 6 O 6 . It is primarily used to create nutty flavors in foods. It is also a common intermediate in the Kreb’s cycle when Citrate is changed into D-isocitrate. You can find detailed UV spectra of Aconitic acid , trans-Aconitic acid and information about its various lambda maxima by visiting the following link.
Aconitic acid , trans-Aconitic acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 75/25% |
|---|---|
| Buffer | Ammonium acetate pH 5.0 – 210 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ESI-SIM: [M-H]1-173, UV 230 |
| Limit Of Detection* | LC MS 100 ppb, UV 28 ppb |
| Class of Compounds | Acid, Carboxylic acids, Tricarboxylic acids |
| Analyzing Compounds | Aconitic acid,trans-Aconitic acid |