HPLC Method for Analysis of Acetaminophen on Primesep 100 Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetaminophen (Paracetamol)
Acetaminophen , also known as Paracetamol, is a medication with the molecular formula C 8 H 9 NO 2 . It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.
Acetaminophen (Paracetamol) can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | Acetaminophen (Paracetamol) |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended