HPLC Method for Analysis of Acesulfame K on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Acesulfame K .
Acesulfame K , also known as Acesulfame potassium and Ace K, is a sugar substitute with the formula C 4 H 4 KNO 4 S . It is significantly sweeter than other popular sugars, but has a bitter aftertaste at high concentration. You can find detailed UV spectra of Acesulfame K and information about its various lambda maxima by visiting the following link.
Acesulfame K can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and sulfuric acid. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 40/60% |
|---|---|
| Buffer | H2SO4– 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 192 nm |
| LOD* | 3 ppb |
| Class of Compounds | Sugar |
| Analyzing Compounds | Acesulfame K |
HPLC Column Used
Newcrom BH, 3.2 x 150 mm, 5 µm, 100 A, dual ended