HPLC Method for Analysis of Abaloparatide on BIST B+ Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Abaloparatide
Abaloparatide is a 34-amino acid parathyroid hormone-related protein (PTHrP) analogue with the molecular formula C 174 H 299 N 56 O 49 . It is used to treat osteoporosis in postmenopausal women who are intolerant to other available osteoporosis therapy. It is also used to increase bone density in men with osteoporosis.
Abaloparatide can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 70-40% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| LOD*UV | 180 ppb |
| Class of Compounds | Protein Analogue |
| Analyzing Compounds | Abaloparatide |
HPLC Column Used
BIST B+, 4.6 x 50 mm, 5 µm, 300 A, dual ended