HPLC Method for Analysis of 6-Benzylaminopurine on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Benzylaminopurine
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C 12 H 11 N 5 . It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.
6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | H2SO4-0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 272 nm |
| Class of Compounds | Synthetic Cytokinin |
| Analyzing Compounds | 6-Benzylaminopurine |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended