HPLC Method for Analysis of 5-Aminoindole on Primesep 100 Column on Alltesta™
https://sielc.com/hplc-method-for-analysis-of-5-aminoindoleChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 5-Aminoindole
5-Aminoindole is an organic compound with the formula C 8 H 8 N 2 . It is a derivative of indole that is primarily used as a building block in the synthesis of various pharmaceutical compounds. You can find detailed UV spectra of 5-Aminoindole and information about its various lambda maxima by visiting the following link.
5-Aminoindole can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with ammonium formate as a buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN -55% |
|---|---|
| Buffer | H2SO4– 0.5% |
| Flow Rate | 1.1 mL/min |
| Detection | UV 275 nm |
| Class of Compounds | Organic Compound |
| Analyzing Compounds | 5-Aminoindole |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended