HPLC Method for Analysis of 4-ethoxyaniline on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Ethoxyaniline .
4-Ethoxyaniline , also known as p-Phenetidine , is one of three Phenetidine isomers, with the chemical formula C 8 H 11 N O . It is commonly used as an intermediate product in drug and dye synthesis. You can find detailed UV spectra of 4-Ethoxyaniline and information about its various lambda maxima by visiting the following link.
4-Ethoxyaniline can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Sulfuric Acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 40% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Aromatic amine |
| Analyzing Compounds | 4-Ethoxyaniline |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended