HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100 Column with Cromite™
https://sielc.com/hplc-method-for-analysis-of-4-amino-2-nitrophenol-cromiteChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Amino-2-nitrophenol
4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C 6 H 6 N 2 O 3 . It is a red-purple powder that is typically used in as an oxidation base in hair dye. California considers this compound a carcinogen.
4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 50% |
|---|---|
| Buffer | Sulfuric Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Phenol Derivative |
| Analyzing Compounds | 4-Amino-2-nitrophenol |
HPLC Column Used
Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended